An artistic representation of the minute nucleic differences detectable using the form of nuclear quadrupolar resonance described in the new paper. Credit: Mathieu Ouellet. CC BY-SA.

A new quantum sensing technology that allows the detection of individual nuclei will open doors in drug development and protein folding.

Since the 1950s, scientists have used radio waves to uncover the molecular ‘fingerprints’ of unknown materials, aiding in tasks as varied as scanning the human body with MRI machines and detecting explosives at airports.

These methods, however, rely on signals averaged from trillions of atoms, making it impossible to detect tiny variations between individual molecules.

Such limitations hinder applications in fields like protein research, where small differences in shape control functionality and can determine the difference between health and disease.

Quantum sensitivity

Now, engineers at the University of Pennsylvania School of Engineering and Applied Science (Penn Engineering) have utilised quantum sensors to realise a groundbreaking variation of nuclear quadrupolar resonance (NQR) spectroscopy, a technique traditionally used to detect drugs and explosives or analyse pharmaceuticals.

The new method is so precise that it can detect the NQR signals from individual atoms — a feat once thought unattainable.

This unprecedented sensitivity opens the door to breakthroughs in fields like drug development, where understanding molecular interactions at the atomic level is critical.

Senior author Lee Bassett, associate professor in electrical and systems engineering (ESE), and director of Penn’s Quantum Engineering Laboratory (QEL), said: “This technique allows us to isolate individual nuclei and reveal tiny differences in what were thought to be identical molecules.

“By focusing on a single nucleus, we can uncover details about molecular structure and dynamics that were previously hidden.

“This capability allows us to study the building blocks of the natural world at an entirely new scale.”

An unexpected discovery

The discovery stemmed from an unexpected observation during routine experiments.

Alex Breitweiser, a recent doctoral graduate in Physics from Penn’s School of Arts & Sciences and the paper’s co-first author, who is now a researcher at IBM, was working with nitrogen-vacancy (NV) centres in diamonds — atomic-scale defects often used in quantum sensing — when he noticed unusual patterns in the data. 

The periodic signals looked like an experimental artifact, but persisted after extensive troubleshooting.

Returning to textbooks from the 1950s and ’60s on nuclear magnetic resonance, Breitweiser identified a physical mechanism that explained what they were seeing, but that had previously been dismissed as experimentally insignificant. 

Advances in technology allowed the team to detect and measure effects that were once beyond the reach of scientific instruments.

Breitweiser said: “We realised we weren’t just seeing an anomaly, we were breaking into a new regime of physics that we can access with this technology.”

Unprecedented precision

Understanding of the effect was further developed through collaboration with researchers at Delft University of Technology in the Netherlands, where Breitweiser had spent time conducting research on related topics as part of an international fellowship.

Combining expertise in experimental physics, quantum sensing and theoretical modelling, the team created a method capable of capturing single atomic signals with extraordinary precision.

The paper’s other co-first author Mathieu Ouellet, a recent ESE doctoral graduate, said: “This is a bit like isolating a single row in a huge spreadsheet; traditional NQR produces something like an average — you get a sense of the data as a whole, but know nothing about the individual data points.

“With this method, it’s as though we’ve uncovered all the data behind the average, isolating the signal from one nucleus and revealing its unique properties.”

Deciphering the signals

Determining the theoretical underpinnings of the unexpected experimental result took significant effort.

Ouellet had to carefully test various hypotheses, running simulations and performing calculations to match the data with potential causes.

He said: “It’s a bit like diagnosing a patient based on symptoms; the data points to something unusual, but there are often multiple possible explanations.

“It took quite a while to arrive at the correct diagnosis.” 

Looking ahead, the researchers see vast potential for their method to address pressing scientific challenges.

By characterising phenomena that were previously hidden, the new method could help scientists better understand the molecular mechanisms that shape our world. The research is published in Nano Letters.